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RP-HPLC Method for Quantitative Estimation of Halquinol in Pharmaceutical Dosage Forms


Nischal Kandepu, Sharadamma C. Kodaganur, Abhilekha P. Mantri, Sarmistha Saha, Radhakrishna M. Pallipadi

Article (PDF)

Keywords

Halquinol; RP-HPLC; Suspension; Bolus

Abstract

Halquinol is a mixture obtained by chlorinating 8-Hydroxy quinoline. It contains 5,7-dichloroquinolin-8-ol (57 to 74%), 5-chloroquinolin-8-ol (23 to 40%), and 7- chloroquinolin-8-ol (up to 4%). It is an antibacterial, antifungal and antiprotozoal in nature, and is used in many areas of the world in poultry and swine rearing to fight microbial infections and in growth promotion. A simple, rapid, economic, accurate reverse phase isocratic HPLC method was developed for quantification of Halquinol in different dosage forms such as suspension and bolus. The method was validated with respect to linearity, precision, accuracy and robustness as per the International Conference on Harmonisation (ICH) guidelines. The calibration curve was linear in the range of 12-28 μg mL-1 for 5-chloroquinolin-8-ol and 33-77 μg mL-1 for 5,7-dichloroquinolin-8-ol respectively. The method has been successfully applied for the analysis of Halquinol in pharmaceutical dosage forms.