Spectrophotometric Method for Determination of Some Third Generation Cephalosporins Antibiotics Using Sodium Molybdate

M. Zaky, S. Fadel, Dr. Khaled Mansour Elgendy*

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Spectrophotometric Method, Cephalosporins, Sodium Molybdate, Pharmaceutical Samples, Urine Sample, Blood Sample


A simple, rapid and sensitive spectrophotometric method has been developed for the quantitative determination of four drugs of pharmaceutical interest; cefotaxime Na, cefoperazone Na, ceftazidime pentahydrate, ceftriaxone Na, 3rd generation cephalosporins The method based on the reduction of the chromogenic agent, sodium molybdate (Mo6+) 'into molybdenum blue (Mo5+) the examined drugs in sulphuric acid medium and by aid of heating in boiling water bath. The resulting "blue colored" products possess a characteristic max at 680 nm, different factors are studied such as, effect of temperature, time, surfactant and interference. Beers law is obeyed over the concentration range of 2 - 70 μg ml-'with molar absorpitivities ranging from 0.599 ×104 Lmol-1cm-1 - 1.794×104 Lmol- 1cm-1and Sandell sensitivities ranging from 0.02538 μg cm-2 - 0.1077μg cm-2. The proposed method has been applied successfully for the determination of the examined drugs both in pure form and in pharmaceutical formulations. The method also finds a good application for urine and blood samples.