Isolation of Heavy Metal Resistant Bacteria from Estuarine Environs in Southeast Coast of India
Balasubramanian R, Jaganathan K, Kavitha V, Ananthan R
Water, Sediment, Physical parameters, Medium, Bacteria, Isolation, THB, HMRB.
Microbial communications with metals may have a few ramifications for the earth. Microbial Organisms may assume a substantial job in the biogeochemical cycling of lethal heavy metals likewise in tidying up or remediating metal-debased conditions. There is likewise proof of a relationship between's resilience to substantial metals and anti-infection opposition, a worldwide issue as of now compromising the treatment of contaminations in plants, creatures, and humans. Samples were made from both stations at weekly interval for a period of three months (August –October 2009) and total of 12 samples were taken for the analysis. Nutrient agar medium were used to estimate the bacterial density. Heavy metals joined media were utilized for the specific confinement of substantial metal resistant bacteria. The concentration of every heavy metal Cu2+ and Fe2+ was kept up from 10ppm to 50 ppm at 10 pmm interims in the way of life medium. The cadmium from 0.5 to 2.5ppm was maintained in the culture medium. Salinity was ranged from 32 to 18 ppt and pH was varied from 8.2 to 7.4 in these stations. THB population were biochemically identified with five species up to genus level of water and sediment in both stations. Maximum of temperaure (340C) was observed at the Vellar estuary in the month of Aug. 2009. Minimum of 180C was observed at Uppnar estuary in the month of Oct 2009. The THB colonies ranged from 3.581X10-9 CFU/ml to 4.325 CFU/ml in water samples at Station 1. The THB colonies varied from 5.712X10-9 CFU/g to 6.971 CFU/g was recorded in sediment samples at Station1. The THB colonies ranged from 2.4 X10-9 CFU/ml to 6.1 CFU/ml in water samples at Station 2. The THB colonies varied from 2.6 X10-9 CFU/g to 9.9 CFU/g was recorded in sediment samples at Station2. The maximum density of HMRB were observed 10 ppm of Cu, Fe and 0.5 ppm of Cd in both water and sediment samples at both stations. A few microbial bacteria can oppose the heavy metals at high poisonous dimensions and the obstruction might be interceded by hereditary elements, creation of chelating operators, authoritative by cell surface slime or potentially oxidative detoxification.