Spectrophotometric Determination of Ranitidine Hydrochloride Based on the Reaction with p-Dimethylaminobenzaldehyde

Badiadka Narayana, Krishnamurthy Ashwini, Divya Narayana Shetty and Kunnummel Veena

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Ranitidine hydrochloride, p-Dimethylaminobenzaldehyde, Spectrophotometry


A simple and sensitive spectrophotometric method is described for the determination of ranitidine hydrochloride (RNH) in pharmaceuticals. It is based on the formation of colored condensation product with p-dimethylaminobenzaldehyde (PDAB) followed by measurement of absorbance at 503 nm. The absorbance was found to increase linearly with the concentration of the drug and formed the basis for quantification. The calibration graph was linear from 50.00 to 350.00 μg mL-1. The apparent molar absorptivity is calculated to be 0.311×104 L mol-1cm-1and the calculated sandell’s sensitivity is 0.1132 μg cm-2. The limits of detection and quantification are found to be 0.00346 and 0.0105 μg mL-1 respectively. The procedure is used to determine ranitidine hydrochloride in pharmaceutical products. The associated pharmaceutical materials did not interfere.