Extraction, Separation and Spectrophotometric Determination of Hg(II) using 2- [(3-hydroxy-4-nitrophenyl) methylidene] hydrazine-1-carbothioamide (HNMHC) as a chromogenic reagent.
DOI:
https://doi.org/10.53555/zyqcf027Keywords:
Mercury (II), HNMHC, spectrophotometric method, environmental samplesAbstract
A simple, selective, and sensitive extractive spectrophotometric technique was successfully developed for the quantitative determination of mercury(II) using 2- [(3-hydroxy-4-nitrophenyl) methylidene] hydrazine-1-carbothioamide (HNMHC) as a chromogenic reagent. Mercury (II) formed a stable complex with HNMHC in the presence of potassium iodide, which was quantitatively extracted in to n-butanol and measured at 368 nm. Experimental parameters affecting complex formation and extraction, including potassium iodide concentration, reagent concentration and extraction solvent were enhanced. Under the optimized condition, beer’s law obeyed over the concentration range of 5-75 μg mL⁻¹ with molar absorptivity of 2.57 × 10⁴ L mol⁻¹ cm⁻¹ and Sandell’s sensitivity of 0.0156 μg cm⁻². The detection limit of the method as 0.0654 μg mL⁻¹. the proposed method shows good selectivity in the presence of several foreign ions, while interference from selected ions was effectively masked. The developed method was successfully applied for the separation and quantification of Hg(II) in binary and ternary synthetic mixtures containing different associated metal ions. The applicability of the method was further demonstrated by analyzing environmental samples, including river water, lake water and groundwater. The developed method is rapid, economical, accurate and suitable for trace level determination of mercury (II)
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