A Quick GC/MS Method correlated with LC/MS/MS for the Identification of Medicinal Natural Products in Convolvulus arvensis: An Injury Healing Plant

Abstract

The identification of the natural products in methanolic extracts of Convolvulus arvensis plant parts was
carried out by using GC/MS and LC/MS/MS chromatographic systems. The study was performed on
both fresh and dry samples. The analysis reveals that many compounds are presented in the extracts.
Some were reported and characterized earlier in several other plants including their biological activities.
Each part of the plant was treated similarly in all steps. The GC/MS chromatograms present peaks that
are major (peak area/total peaks area>0.2%). In the leaves extract, seventeen compounds were detected
as individual, sharp, well resolved and readily quantified peaks. Four peaks are major and identified to
be Neophytadiene, hexadecanamide, 9-octadecanamide and 1,2-benzendicarboxylic acid. In stems
extract, thirteen compounds were observed, four peaks are major: hexadecanamide, 9-octadecanamide,
1,2-benzendicarboxylic acid and stigment-5-en-3-ol. In roots extract, twelve compounds were detected,
three peaks are major: hexadecanamide, 9-octadecanamide and 1,2-benzendicarboxylic acid. The dry
extract contains 25 compounds, eight compounds are major and identified to be Neophytadiene, stearic
acid, hexadecanamide, 9-octadecanamide, 1,2-benzendicarboxylic acid, Vitamin E, stigment-5-en-3-ol
and 5-beta-pregn-7-en-3,20-dione. The LC/MS/MS measurements were performed and the results of the
two chromatographic methods were correlated and found to be consistent. The major compounds are
detected in the two chromatographic methods. The dry and fresh plant was traditionally examined to be
used for treatment of different ailments. This study was performed because the fresh and dry
Convolvulus arvensis were used for the treatment of skin cuts or wounds, to stop bleeding and promote
quick injury healing.