Evaluation of Antioxidant Activity of Armillaria tabescens, Leucopaxillus gentianeus and Suillus granulatus: The mushroom Species from Anatolia

Abstract

The antioxidant activity of extracts of Armillaria tabescens, Leucopaxillus gentianeus and
Suillus granulatus were determined for the first time by using five complementary tests;
namely, β-carotene-linoleic acid, DPPH•
scavenging, ABTS•+ scavenging, metal chelating and
CUPRAC assays. In DPPH•
scavenging, ABTS•+ scavenging and CUPRAC assays, the ethyl
acetate extract of S. granulatus showed the best activity 91.52±0.97%, 89.67±0.15% and
3.90±0.09 at 400 µg/mL concentration, respectively while in β-carotene-linoleic acid assay, the
methanol extract of this mushroom exhibited higher activity. In addition, among the extracts of
mushroom species, particularly the hexane extracts showed better activity in metal chelating
activity. The hexane extract of L. gentianeus exhibited the highest metal chelating activity
(70.68±0.34%) at 400 µg/mL concentration. In conclusion, the results showed the antioxidant
importance of the studied mushroom species, growing naturally in Anatolia. Thus, particularly
S. granulatus may have been helped people to protect against lipid peroxidation and free radical
damage, and its extracts will probably use for the development of safe food products and
additives.