Spectrophotometric Simultaneous Determination of Rabeprazole Sodium and Domperidone in Combined Tablet Dosage Form

Abstract

A simple, economical, precise and accurate method for simultaneous determination of
Rabeprazole Sodium (RAB) and Domperidone (DOM) in combined tablet dosage form has been
developed. The method is based on Ratio Spectra Derivative Spectrophotometry. The
amplitudes in the first derivative of the corresponding ratio spectra at 230 nm and 250 nm were
selected to determine Rabeprazole Sodium and Domperidone respectively. Beer’s law is
obeyed in the concentration range of 10-30 μg mL-1
for RAB and 5-15 μg mL-1
for DOM. The %
assay for commercial formulation was found to be 99.82  0.552 for RAB and 99.63  0.791 for
DOM. The method was validated with respect to linearity, precision and accuracy.