Synthesis a New Schiff Base as a Chelating Agent for Reliable Quantification of Zinc from Water and Biological Samples

Abstract

A new Schiff base, 2-[(4-Chloro-2-oxo-2H-chromen-3-ylmethylene)-amino]-propionic
acid was successfully synthesized and characterized by proton nuclear magnetic
resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR), Fourier
transform infrared (FT-IR), elemental analysis (CHN) and UV–Vis spectroscopy. It was
found out that the synthesized Schiff base could be used as a novel and reliable
candidate to detect and quantify of Zn (II) ions in water and biological samples. Some
effective parameters on quantification of Zn (II), such as pH, Schiff base and salt
concentration were inspected by a (23) full factorial design using Design-Expert,
software. Under optimum conditions, the calibration graph was linear in the range
0.04–10.0 μg mL−1 with a limit of detection of 0.01 μg mL−1. The proposed method was
successfully applied to the rapid determination of Zn (II) in water and biological
samples. The validation was performed by inductively coupled plasma atomic emission
spectrometry (ICP-AES) with satisfactory results.