Determination of Albendazole in the Presence of its Alkaline Degradation Product Using TLC-Densitometric and Chemometric Methods: A Comparative Study

Abstract

Four simple, specific, accurate and precise methods have been used for the determination
of albendazole (ALB) in the presence of its alkaline degradation product. Method A, a
densitometric one, after separation on silica gel plate using dichloromethane: methanol
(90:10 v/v) as a mobile phase and the spots were scanned at 232 nm. Beer’s law was obeyed
over the concentration range of 1–5 μg/spot. Methods B, C and D, chemometric-assisted
methods, including classical least squares (CLS), principal component regression (PCR) and
partial least squares (PLS), albendazole was analyzed with mean accuracy of 99.940.159,
99.930.159 and 99.930.158 for CLS, PCR and PLS, respectively. A comparative study
between the methods was done showing the advantages and the disadvantages of each
method. These methods are suitable as stability indicating methods for the simultaneous
determination of albendazole in the presence of its alkaline degradation product either in
bulk powder or in pharmaceutical formulations