HPLC Profiles of Dipterocarpus crinitus Extracts from Different Plant Organs and Geographical Locations

Abstract

Dipterocarpuscrinitus is a timber tree that belongs to Dipterocarpaceae family.
Dipterocarpaceae is found to contain oligostilbenes, a polyphenolic group that have been
widely studied as they show biological activities. To date, about one-fourth of all stilbene
derivatives reported were isolated from Dipterocarpacae plants. Chromatographic profiles
of 23 samples of D. crinitus extracts were recorded on UHPLC system. For all samples, the
wavelengths were set at 215nm, the column temperature was 39C, the flow-rate was 1
ml/min and the injection volume was 5 l. The mobile phase was a gradient of acetonitrile:
water (ACN: H2O) 5:95 to 55:45 in a chromatographic run that was standardized for 15
minutes. The samples were of bark and wood from both trunk and buttress of D. crinitus
collected from Selai, Jengka and Keniam. Analysis of all chromatograms showed that
samples from the same species having similar chromatographic profiles regardless their
plant parts and localities. In contract, extracts from different species show significant
difference in their chromatographic patterns and composition of compounds. This study
showed that the use of different HPLC techniques is an effective method to determine the
differences and similarities of samples containing closely related chemical compositions.