Liquid chromatographic method for determination of moxifloxacin and dexamethasone sodium phosphate in eye drops

Abstract

A liquid chromatographic method was developed and validated for the simultaneous determination of
moxifloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations.
Optimum separation was achieved in less than 5 min using a C18 column (250 mmx4.6 mm i.d, 5µ
particle size) by isocratic elution. The mobile phase consisting of a mixture of 0.01M phosphate buffer
(pH 6.0) and acetonitrile (50:50, v/v) was used. Column effluents were monitored at 254 nm at a flow
rate of 1 mL min-1. Retention times of moxifloxacin and dexamethasone sodium phosphate were 2.58
and 3.95 min respectively. The linearity of moxifloxacin and dexamethasone sodium phosphate was in
the range of 10-60 µg mL-1 and 2-12 µg mL-1 respectively. Developed method was economical in
terms of the time taken and amount of solvent consumed for each analysis. The method was validated
and successfully applied to the simultaneous determination of moxifloxacin and dexamethasone
sodium phosphate in bulk and pharmaceutical formulations.