Spectrophotometric Method for Determination of Trimetazidine in Formulation Using Chloranil as Chromogenic Agent

Abstract

A new, simple, selective and more reproducible spectrophotometric method for the determination of
trimetazidine (TMZ), a piperizinyl antianginal has been developed and validated. This method was
based on the formation of a blue colored chromogen between trimetazidine and chloranil acetaldehyde
reagent in DMSO. The chromogen exhibit maximum absorbance at 627 nm with molar absorptivity of
5.205 x 104
lit mol-1
cm-1
in methanol. Beer’s law was found to be obeyed in the concentration range
of 30 - 65 µg mL-1 with linear regression of 0.9984, while the percentage recovery and the limit of
detection 98.81-99.91%, 5.5 µg mL-1
respectively. From the percentage recovery and placebo studies
it was concluded that there was no interference of additives during the estimation. This shows the
suitability of this method for the routine analysis of the trimetazidine in tablet formulation.