Rapid FTIR Method for Estimation of Sucrose in a Traditional Indian Polyherbal Formulation

Abstract

A simple, fast and reliable method has been developed for the quantification of sucrose in an Indian
traditional polyherbal formulation samples (Amukkara choornam) through Fourier Transform Infrared
absorbance measurements (FTIR) at 1051 cm-1 with a base line established between 750 cm-1 to 1500
cm-1. This method was validated following the united state pharmacopoeia guidelines for method
validation. Sensitivity, Linearty, Limit of detection (LOD), Limit of quantification (LOQ), Accuracy
and Specificity were considered. Sucrose was used as standard.