Spectrophotometric Determination of Ranitidine Hydrochloride Based on the Reaction with p-Dimethylaminobenzaldehyde

Abstract

A simple and sensitive spectrophotometric method is described for the determination of ranitidine
hydrochloride (RNH) in pharmaceuticals. It is based on the formation of colored condensation product
with p-dimethylaminobenzaldehyde (PDAB) followed by measurement of absorbance at 503 nm. The
absorbance was found to increase linearly with the concentration of the drug and formed the basis for
quantification. The calibration graph was linear from 50.00 to 350.00 μg mL-1. The apparent molar
absorptivity is calculated to be 0.311×104
L mol-1cm-1and the calculated sandell’s sensitivity is 0.1132
µg cm-2. The limits of detection and quantification are found to be 0.00346 and 0.0105 µg mL-1
respectively. The procedure is used to determine ranitidine hydrochloride in pharmaceutical products.
The associated pharmaceutical materials did not interfere.