A Stability-Indicating HPLC Method for Cefoperazone

Abstract

Stability indicating HPLC assay method for the standard drug cefoperazone was developed using reverse phase
appurassil C-18, 250 X 4.6mm. 5µm column, in the mobile phase phosphate buffer (pH 6.8) and methanol (5:2)
at flow rate 1mLmin-1 with UV detection at 254 nm. The retention time was found to be 2.67 min. Validation of
an analytical method was established by laboratory studies. The proposed method was found to be linear at
concentration of 1 to 10 µgmL-1 (R2
=0.9899). The limit of detection and limit of quantification was 0.2 µgmL-1
and 0.4 µgmL-1 respectively and the method was found to be specific. Method precision and precision of the
system was found to be within the limits of the acceptance criteria. Relative Standard deviation for precision of
the method and precision of the system was found to be 0.49% and 0.7451% respectively. The percentage
recovery ranges from 95 –106 % The results indicate that there is no interference from excipients for the
proposed method, thus making the method more simple, less time consuming and suitable for routine
quantitative estimation of cefoperazone sodium injection formulation. As the method could effectively separate
the drug from its degradation products, it can be employed as a stability indicating one