Differential Pulse Voltammetric Determination of Penicillin G at Boron Doped Diamond Electrode
Keywords:
Differential Pulse Voltammetry, Boron Doped Diamond Electrode, Penicillin G.Abstract
Concerns about accumulation of persistent pollutants such as penicillin G (PG) in
aquatic environments results in the demand for analytical methods suitable for their fast,
sensitive and simple determination. This study assesses the effectiveness of differential pulse
voltammetric (DPV) method coupled with boron doped diamond electrode (BDDE) for
determination of PG in aquatic samples. Britton-Robinson buffer (BRB) was used as a
supporting electrolyte. Results showed that the DPV technique is suitable for the
determination of PG in both drinking and river water model samples. PG gives an anodic DPV
peak in the range from +800 to +1010 mV, depending on the pH of BRB. The best developed
peak was obtained in BRB of pH 4, at the potential of +900 mV. Calibration curves are linear
(R>0.99) in the range of 1- 9 µM of PG with the limit of quantitation (LOQ) of 1.5 µM and the
limit of detection (LOD) of 0.23 µM. The method is not influenced by inorganic ions present in
measured solutions, there are no problems with electrode passivation by products of PG
anodic oxidation and it is accurate (RSD of 1.7-2.3%). Our developed is used as a simple,
accurate, reliable and robust method for PG determination.