One and Two‐dimensional Nuclear Magnetic Resonance Spectroscopy Analysis of Enoxaparin Samples

Abstract

Heparin has been used as a clinical anticoagulant for more than 50 years and it
has been the second most used drug in the world. Unfractionated heparin are broke down
by either specific lyase enzymes or chemical depolymerizations to obtain various of low
molecular weight heparins (LMWHs) maintains much of the antithrombotic activity and
provides a more predictable pharmacokinetic profile and a ease of use. Although this
process is believed to be well regulated and inspected, LMWHs may still show distinct
pharmacological and biochemical profiles primarily because of their structural differences.
These differences which derived from LMWHs preparation process can be accurately and
precisely identified by nuclear magnetic resonance (NMR) spectroscopy. This study is
designed to compare the structural differences of 11 enoxaparin copies from different
manufacturers. Using a combination of one‐ and two‐dimensional high sensitivity NMR, we
were able to accurately integrate the monosaccharide residues of LMWHs and exhibit the
structural differences