Simultaneous Determination of Levetiracetam and Preservatives in Oral Solution Formulation Using Hplc-Uv Method with a Programble Detection Wavelength

Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method
was developed and validated for the simultaneous determination oflevetiracetam,
methylparaben and propylparaben in levetiracetam oral solution formulation. The
separation was achieved on Equisil BDS, C18, 5 µm, (150 mm × 4.6 mm) using 1.4 g/L of
NaH2PO4 : Methanol in ratio (55:45) respectively pH 7.7 by NaOH as mobile phase and at a
flow rate of1.0 mL/min. Detection was carried out using a UV detector Start with 240 nm
then at 9 minutes change to 254 nm. The total chromatographic analysis time per
samplewas about 14min. Analytical parameters system suitability, specificity, linearity,
precision, repeatability accuracy, LOD/LOQ and stability of standard solution were
determined by validation procedure and found to be satisfactory