Prebiotic Effect of Cissusquadrangularis and Probiotic Potential against V.parahaemolyticus Isolated from Shrimp Cultivation Pond

Abstract

In the present study, the prebiotic potential of Cissusquadrangularisand
immobilization ofLAB wasinvestigated against V.parahaemolyticus. The effectiveness of
probiotic activity was determined by coaggregation, hydrophobicity and biofilm formation
capability of both immobilized and native LAB. It was noted that the Poly saccharide
ofCissusquadrangularis acted as prebiotic and stimulate or sustain the viability of LAB under
chitosan entrapment. Immobilization of Lactobacillusspshowed effective autoaggregation at
the 5 hour by immobilized cell and 24 hour for native LAB.It was noted that entrapment of
LAB with natural prebiotic and chitosan polymer have had potent probiotic properties with
improved hydrophobicity, coaggregation as well as a strong biofilm production
onLactobacillus sp.Immobilized LAB have had a coaggregation 82% and 74% respectively on
chitosan and alginate