ANTIMICROBIAL METABOLITE OF ZINGIBERACEAE ESSENTIAL OILS USING RESAZURIN A RAPID COLORIMETRIC DETECTION

Abstract

An antimicrobial assay using broth microdilution is the method accepted and approved
by Clinical and Laboratory Standards Institute (CLSI). This technique was in vitro and minimized a
volume of the medium that was performed the susceptibility of antimicrobial activity using
resazurin rapid colorimetric detection. The technique had performance testing antimicrobial
activities of essential oil from Zingiberaceae; Curcuma longa L, C. zedoaria (Christm.) Roscoe, C.
aromatica Salisb, C. comosa Roxb., and Zingiber cassumunar Roxb. An approval this technique was
confirmed using agar disc diffusion assay. All the essential extracted had bioactives against all
testing pathogenic gram positive and gram negative bacteria and infectious yeast Candida albicans
especially C. aromatica and C. comosa had inhibited Pseudomonas aeruginosa more than
Ampicillin, antibacterial drug. Hence, it is expected that, from these results, C. aromatica and C.
comosa rhizomes from different habitats should be used more rationally and effectively as natural
pharmaceutical, culinary and cosmetic additives in the future.