In vitro α-Glucosidase and α-Amylase Enzyme Inhibitory Effects in Elaeagnus angustifolia Leaves Extracts

Abstract

This study was performed to demonstrate α-glucosidase and α-amylase enzyme inhibitor
activities using the methanolic extracts of Elaeagnus angustifolia leaves. Methanolic extracts
were prepared by two different extraction methods [accelerated solvent extractor (ASE) and
Soxhlet apparatus]. The analytical procedure involved the application of liquid
chromatography. Our results showed that the ASE extracts inhibited α-amylase (10-100 µg/
ml) and α-glucosidase (10-50 µg/ml) dose-dependently exhibitedrp inhibitory activities
with the extracts [α-amylase (40%) and α-glucosidase (56%)]. RP-HPLC analyses of the
methanolic extracts were detected with vanillic acid and 4-hydroxybenzoic acid as the main
phenolic acids in all the ASE extracts. Elaeagnus angustifolia leaf extracts may be attributed
to the presence of vanillic acid and 4-hydroxybenzoic acid, there by confirming with its
traditional use for the management of diabetes mellitus. We implicate that the anti-diabetic
activity through in vitro assessments and support to the folkloric use of this plant for
controlling type-2 diabetes mellitus in Turkey