Synthesis of Self-Assembled Hydroxyapatite Membrane Using Bio-Template with Surface Modification
Keywords:
Eggshell membrane, hydroxyapatite, scaffold, self assembly, and simulated body fluid.Abstract
Many researches in tissue engineering have been conducted continuously to develop the
proper synthetic materials for tissue and organ substitute materials. At this research, the synthesis
of hydroxyapatite membrane for bone scaffold application has been done successfully by self
assembly method using eggshell membrane with surface modification as a template. By this
method, the pores obtained will be able to resemble the cells microenvironment thus can enhance
interface interaction and support cell proliferation. Before incubating in modified Simulated Body
Fluid (SBF) Kokubo solution, eggshell membrane surface is modified using alginate and chitosan
solutions to increase the number of hydroxyapatite particles bonded due to enhance the
bioactivity of scaffold. The Scanning Electron Microscopy (SEM) result showed that
hydroxyapatite membrane has been formed on scaffold’s surface where the diameter of fibers had
thickened to 2.035, 2.591 and 1.408 μm and particle size of 1.021, 0.934 and 0.895 μm for 6, 9 and
12 days incubation time respectively. It confirmed that the number of hydroxyapatite grown
increases along with the incubation time. These results are in good agreement with FT-IR analysis
that indicates the presence of PO43- functional group on the scaffolds.