An Experimental Study On In Vitro Antioxidant And Antimicrobial Activity Of Ethanolic Extract Of Brassica Oleracea Leaves

An Experimental Study On In Vitro Antioxidant And Antimicrobial Activity Of Ethanolic Extract Of Brassica Oleracea Leaves


  • Mohit Kotnala
  • Kapil Raghuwanshi
  • Neha Singh
  • Pratima Shinde
  • Anil Kumar


Brassica oleracae, Ofloxacin, Antioxidant, Antimicrobial, In-vitro


Green cabbage, or Brassica oleracae, is a herbaceous biennial plant whose leaves assemble into a compact head. It is an edible vegetable that has long been used as a medicinal herb, with several health advantages claimed. The purpose of this study is to evaluate the extract of Brassica oleracea leaves for antioxidant and antibacterial properties. Brassica oleracea leaf hydroalcoholic extract was tested for antioxidant activity using a variety of in vitro models, including the 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) assay. In the investigated models, the extract exhibited dose-dependent properties related to free radical scavenging. The DPPH technique yielded an IC50 value of 78.19 μg/ml for the extract of Brassica oleracea leaves, which was similar to ascorbic acid (IC50=18.78 μg/ml). The medication used in the usual formulation was IP-grade ofloxacin and ofloxacin. Escherichia coli and Streptococcus mutans were cultured for 24 hours in order to test the antibacterial activity. Three concentrations of extracted phytochemicals—25, 50, and 100 mg/ml—were employed in antibiogram experiments. One important purpose is to promptly insert wells containing antibiotics on the agar surfaces following the organism under study's inoculation. This research defines Brassica oleracea's antioxidant and antibacterial properties, which will be used to medicine in the future.

Author Biographies

Mohit Kotnala

Science Teacher, Bloom Charter Education, Ain Al Fayedah School-6021 Abu Dhabi United Arab Emirates.

Kapil Raghuwanshi

Assistant Professor, Department of Biochemistry, Chhindwara Institute of Medical Sciences, Chhindwara, Madhya Pradesh, India

Neha Singh

Assistant Professor, Department of Pharmaceutical Chemistry, Axis Institute of Pharmacy, Kanpur, Uttar Pradesh, India

Pratima Shinde

Associate Professor, Department of Pharmaceutics, Siddhant College of Pharmacy, Sudumbare, Pune, India

Anil Kumar

Assistant Professor & Head,  Department of Chemistry (PG), Sahibganj College Sahibganj,  Jharkhand, India


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