A Stability-Indicating Reverse Phase High Performance Liquid Chromatography Method For Development And Validation Of Meptazinol
Keywords:
Reverse phase high-performance liquid chromatography, UV-visible spectroscopy, method development, validation, meptazinol, international conferenceAbstract
Methodology: The UV-Spectroscopic method was developed for the estimation of meptazinol in bulk and tablet dosage form. The solvent selected for the meptazinol UV analysis was methanol, the solution of 10µg/ml was scanned in UV region from 200-400 nm and the λmax value was determined. The RP-HPLC method was developed on HiQ silC18HS (250mm x 4.6 mm, 5 )column using acetonitrile: methanol [90:10] as mobile phase at flow rate 1.2 ml/min and UV detection at 276 nm. Results: The maximum absorbance was observed at 276 nm. The wavelength 276 nm was selected for further analysis of Meptazinol . The calibration curve was determined using drug concentrations ranging from 10-60 µgm/ml. The system suitability was performed by injecting a standard solution containing 60µg/ml of meptazinol six replicates. For two of them, the peak asymmetric were 2000, and the %RSD of meptazinol was less than 2.
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