SEGREGATION AND STRUCTURAL INTERPRETATION OF NOVEL TETRASACHHARIDES “ISOSE” BY NMR AND MASS SPECROSCOPY

Abstract

Carbohydrate are abundantly found in milk and precursor for many organic
molecules like amino acids, fats etc. Milk is principal source of nutrition in infant. It is full of
bioactive molecules, which act as anti-viral, anti-bacterial, anti-inflammatory, anti-cancer, etc. In
traditional medine, “Aurveda and Unani System” of medicine it bother hiccup and dyspnoea, it
rises ‘pitta and kapha’ and decreases body fat. Keeping in mind these biological activity, Gaddi
sheep’s milk is processed by Kobata and Ginsberg method after that gel filtration, HPLC and
column chromatography, which result isolation of novel oligosaccharide named Isose. The
structure of isolated and purified Isose was decoded with the help of chemical degradation,
chemical transformation and different spectroscopic methods like- NMR (1H, 13C, COSY, TOCSY,
HMBC, HSQC etc.), and Mass spectroscopy. The structure of isolated tetrasaccharide “Isose” is
explained as-
GlcNAc(16)GalNAc(14)GalNAc(14)Glc