Chemometric Assisted Spectrophotometric Methods for Simultaneous Determination of Paracetamol and Tolperisone Hydrochloride in Pharmaceutical Dosage Form

Abstract

Three simple Thermometric assisted UV- Visible Spectrophotometric methods, Classical
Least Square (CLS), Partial Least Square (PLS) and Principal Component Regression (PCR)
were developed for simultaneous estimation of PCM and TOL in pharmaceutical dosage
form without any chemical separation and any graphical treatment of the overlapping
spectra of two drugs. The UV absorption spectra of the drugs studied in the range of 220 –
280 nm. Beer’s law was obeyed for both drugs in the concentration ranges of 5 – 25 µg/ml
for Paracetamol and 1.5 – 7.5 µg/ml for Tolperisone Hydrochloride. Twenty five (25) mixed
solutions were prepared for the chemo metric calibration as training set and sixteen mixed
solutions were prepared as validation set. The absorbency data matrix was obtained by
measuring the absorbency at twenty-one wavelength points, from 220 to 280nm with the
interval of 3nm. The developed methods can be applied in simultaneous determination of
the selected drugs from the pharmaceutical formulation in routine analysis.