Design of Experiments Based Validated HPTLC Method for Quantification of Oxybenzone and Avobenzone in Personal Care Products

Abstract

A rapid, simple and economic High Performance Thin Layer Chromatography (HPTLC)
method for the simultaneous quantification of Oxybenzone and Avobenzone in various
personal care products available in local markets in and around Chennai is developed and
validated. Central Composite design (CCD) with three factors and one response was
employed to study the robustness of the developed method. The best chromatographic
separation was achieved employing Toluene: Acetonitrile: Ethyl acetate (4: 5: 1 v/v/v) as
solvent system. Densitometric analysis was carried out at 332 nm. The developed method
is examined for validation parameters like linearity, specificity, precision, accuracy and
robustness. The method was found linear over a wide range of 2.5 – 200 ng/band with a
regression coefficient of 0.9998 and 0.9991 for Oxybenzone and Avobenzone respectively.
All the validation parameters were found to be within the acceptance limit. The proposed
and developed HPTLC method can be applied for routine analysis of Oxybenzone and
Avobenzone, in the presence of other excipients in cosmetic products and can also be
extended to analysis in pharmaceuticals and food products.