Spectrophotometric - Flow Injection Analysis Determination of Hydrogen Peroxide Via Quenching of Bromine Absorbance

Abstract

A proposed method is described for the determination of hydrogen peroxide in
three pharmaceutical samples based on the in situ generation of bromine from the BrO3- -
Br--H3O+ reaction; the bromine is give a continuous absorbance measured by Ayah
3SBGRX3-3D solar cell CFIA microphotometer and then when injecting hydrogen peroxide, it
will quenching the response because bromine molecules are converted to bromide ions.
The linear range of 0.01-15mmol.L-1 along the correlation coefficient (r) of 0.9959, limit of
detection (LOD) of 14.45ng/sample. A comparison was made between two methods: newly
proposed method and the classical method using the standard addition procedure, via the
paired t-test and there was no significant difference between the two methods at 95%
confidence level.