Synthesis, separation and determination of metal dithiocarbamates complexes by HPLC-ICP-MS

Abstract

A rapid, simple and reliable spectroscopic technique has been developed for the determination of
metal cations using dithiocarbamates moiety as chelating agent by HPLC. The method is based on the
formation of stable colored dithiocarbamate complexes by reaction metal salt and dithiocarbamate
moiety, which is then extracted methanol and separated with acetonitrile water mobile phase. The
different analytical parameters affecting the method such as pH for extraction, solvent for extraction,
mobile phase and type of the column. After the column elution the sample was allow to passes in to
UV detector and then into ICP-MS. The separate data have been observed from UV detector and ICPMS for each sample and then from the help of both results the identification and determination of
these complexes have been done. Satisfactory recovery from the spiked samples of standard metal ion
suggests no interference of any excipients. The developed method was compared with other
spectroscopic methods, no significant difference was observed.