Development and Validation of UV Spectrophotometric Method for Determination of Milnacipran in bulk and Pharmaceutical Dosage Form

Abstract

Milnacipran is an antidepressant drug belonging to the class of serotonin and noradrenaline reuptake
inhibitors. A new, rapid, simple and sensitive spectrophotometric method has been developed for the
determination of milnacipran in bulk and pharmaceutical formulations. The linearity was observed in
the concentration range of 2-45 µg mL-1
. The method is based on spectrophotometric determinaiton.
Absorbance of milnacipran was determined at 220 nm wavelength. The method was validated in terms
of accuracy and precision (intra and interday variations). This method is extended to pharmaceutical
preparations. Results of the analysis were validated statistically and by recovery studies. The proposed
method was found accurate, reproducible and economical for the routine analysis of milnacipran in
bulk and pharmaceutical formulation.