Development and Validation of Spectrophotometric Method for Simultaneous Estimation of Perindopril and Indapamide in Combined Dosage Form by Simultaneous Equation Method

Abstract

A new sensitive, simple, rapid and precise spectrophotometric method has been developed for
simultaneous estimation of perindopril and indapamide in pharmaceutical dosage form. This method
was based on UV-spectrophotometric determination of two drugs, using simultaneous equation
method. It involves measurement of absorbances at two wavelengths 210.4nm (λmax of perindopril)
and 241.2nm (λmax of indapamide) in methanol. The linearity was observed in the concentration
range of 24 – 56 µg mL-1
for perindopril and 7.5 – 17.5 µg mL-1
for indapamide. The accuracy and
precision of the method was determined and validated stastically. The method showed good
reproducibility and recovery with % RSD less than 2. Method was found to be rapid, specific, precise
and accurate, can be successfully applied for the routine analysis of perindopril and indapamide in
bulk, and combined dosage form without any interference by the excipients. The method was validated
according to ICH guidelines.