Validation and Uncertainty Estimation of Analytical Method for Determination of Benzene in Beverages

Abstract

Analytical method for the determination of benzene in beverages was developed using HS/GC-FID
technique. Validation of the method was done for knowing the performance of the analytical method
used. Linearity obtained r
2
= 0.9971 was observed in the range from 5 to 100 ng mL-1
of benzene in
sample. The method has good recoveries (average 101 %) and average of relative standar deviation for
repeatability and intermediate precision at 3 level concentrations are less than 10 %. The limit of
detection and limit of quantitation obtained from calculation are 0.90 and 2.86 ng mL-1
respectively.
The expanded uncertainty of the method, U, is about 30 %.