A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form

Keddal G. Lalitha, Thangavel Venkatachalam, Ramaraj Srinivasan, Ponnusamy Kalaiselvi, Nagappan Kannappan

A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form

Authors

Keddal G. Lalitha, Thangavel Venkatachalam, Ramaraj Srinivasan, Ponnusamy Kalaiselvi, Nagappan Kannappan

Keywords:

Diacerein, RP- HPLC, Tablets, Validation

Abstract

A simple, economic, accurate reverse phase isocratic RP-HPLC method has been developed for the
quatitation of diacerein in tablet dosage form. The quantitation was carried out using Zorbax CN
column. The mobile phase was ammonium acetate buffer (pH adjusted to 3.5) : Acetonitrile [53:47].
The LOD and LOQ are found to be 3.952 µg mL-1 and 11.97 µg mL-1 respectively. The flow rate was
1mL/min with UV detection at 254 nm.The method has been validated and proved to be accurate,
precise, linear, rugged, robust, simple and rapid. The calibration curve was linear in the concentration
range 25-150 µg mL-1 with coefficient of correlation 0.99942. The percentage recovery of diacerein
was found to be 101.60%.The method is useful in the quality for the estimation of diacerein in tablet
dosage form

A Simple HPLC Method for Quantitation of Diacerein in Tablet Dosage Form