New RP-HPLC Method for The Determination of Olmesartan Medoxomil in Tablet Dosage Form

Abstract

A simple rapid, sensitive, accurate, precise and reproducible high performance liquid chromatographic
method has been developed to assay Olmesartan medoxomil in tablet dosage form. The HPLC
analysis used a reversed phase Hypersil BDS C8 (250X4.6mm, 5µm) column and a mobile phase
constituted of buffer and acetonitrile (55:45 % v/v). The buffer is composed of 3 g of sodium
perchlorate and 3 mL of tri ethyl amine in 1000 mL of water and the pH of the solution was adjusted
to 3.0 with orthophosphoric acid. The wave length of the detection is 250 nm. The validation data
showed that the assay is sensitive, specific and reproducible for the determination of olmesartan in the
dosage form. The method is linear from 10 µg mL-1 to 120 µg mL-1. The accuracy of the method was
found to be 99.54%. Mean inter and intraday assay relative standard deviation (RSD) were less than
1.0%. The proposed method provided an accurate and precise analysis of olmesartan in its
pharmaceutical dosage form.