GC-MS and AAS in Fractionation Analysis of Herbal Supplement

Abstract

An analytical scheme based on solid phase and liquid-liquid extraction was proposed for
the fractionation of element species and organic constituents in a mixture (1:1) of 8 Bulgarian
medicinal plants proposed as herbal nutritional supplement using GC-MS and AAS. Solid phase
extraction was performed with water, 0.1 mol L
-1 HCl, Tris-buffer (pH 7.6) and methanol. Liquidliquid successive extractions were performed with petroleum ether, diethyl ether, CHCl3 and
ethyl acetate. The potential bioavailability of water and lipid – soluble compounds were
discussed.