Liquid Chromatography with Indirect UV Detection for Determination of Some Inorganic Anions Using a Permanently Coated Column: Determination of Monofluorophosphate, Phosphate and Iodide
Keywords:
Cetylpyridinium chloride, Inorganic anions, Ion chromatography, Permanently coated column.Abstract
A method for the separation and determination of inorganic anions using a reversed
phase column permanently coated with n-cetylpyridinium chloride is described. A 1 mM sodium
salicylate solution in 5% (v/v) methanol was used as the mobile phase. The separation of some
inorganic anions (monofluorophosphate (MFP), phosphate and iodide) was carried out in about
10 minutes. Detection was made using indirect UV detection at 290 nm. Satisfactory
reproducibility of the results was obtained even after a four months period of continual use of
the column. Through the evaluation of the analytical parameters, the method was linear (r =
0.999) at concentrations ranging from 10.0 to 100.0 μg mL-1.