Simultaneous Determination of Paracetamol and Piroxicam in Tablets by Thin Layer Chromatography Combined with Densitometry

Abstract

A simple, rapid and accurate High-performance thin-layer chromatography (HPTLC)
method has been established and validated for the simultaneous determination of paracetamol
and piroxicam in tablets. The method is based on HPTLC separation of the two drugs
followed by densitometric measurements of their spots at 288 nm. The separation was carried
out on Merck TLC aluminium sheets of silica gel 60F-254 using n-Dichloroethane: methanol:
triethylamine (10:2.5:1, v/v) as a mobile phase. Calibration curves were linear in range of 1.625
- 14.625 µg/spot and 0.1 - 0.9 µg/spot for paracetamol and piroxicam, respectively. Method was
successively applied to tablet formulation. No chromatographic interferences from the tablet
excipients were found. The method was validated in accordance with the requirements of ICH
guidelines.