Simultaneous Estimation of Ciprofloxacin Hydrochloride, Ofloxacin, Tinidazole and Ornidazole by Reverse Phase – High Performance Liquid Chromatography

Abstract

A new, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid
chromatography assay has been developed for simultaneous estimation of Ciprofloxacin
Hydrochloride, Ofloxacin, Tinidazole and Ornidazole in tablet formulations. The separation was
achieved by using C-18 column (Phenomenax, 250 x 4.6mm i.d.) coupled with a guard column of
same material, in mobile phase Acetonitrile: Water: Tri ethylamine (25:75). The pH of mobile phase
was adjusted to 6.0 ± 0.1 with 50% ortho phosphoric acid. The flow rate was 1.0 mL.min-1 and the
separated drugs were detected using UV detector at the wavelength of 300 nm. The retention time of
Ciprofloxacin Hydrochloride, Ofloxacin, Tinidazole, and Ornidazole was noted to be 2.7, 3.5, 4.5 and
5.8 min, respectively, indicative of rather shorter analysis time. The method was validated as per ICH
guidelines. The proposed method was found to be accurate, reproducible, and consistent. It was
successfully applied for the analysis of these drugs in marketed formulations and could be effectively
used for the routine analysis of formulations containing any one of the above drugs, or a combination,
without any alteration in the chromatographic conditions.