A 1H-NMR based Method for Quantifying Creatinine in Urine Samples from Human Intervention Studies
Keywords:
anthocyanins, creatinine, 1H-NMR, human interventions, urineAbstract
To overcome problems arising from the coloration of urine samples and the limitations of the picric acid method when determining creatinine in urine, we have established an alternative method using 1H-NMR. The method is practically straight forward, provides good recoveries of 105 ± 2%, and a linear regression yielded an R² value of 1. The method revealed a similar precision to the standard procedure based on the Jaffé reaction for urine samples without interfering components.