Investigation on the in Vitro Biotrans Formation of NCE Using LC-MS/MS

Abstract

With the dramatic increase in the number of new chemical entities (NCEs) arising from combinatorial
chemistry and high-throughput processes, novel bioanalytical techniques are required for the rapid
determination of the metabolites of these NCEs. Knowledge of the metabolic sites of the NCEs in early
drug discovery is essential for selecting compounds with favorable pharmacokinetic. In development,
elucidation of biotransformation pathways of a drug candidate it is important to understand its
physiological effects. Liquid chromatography(LC) coupled with atmospheric pressure ionization (API) MS
has now become the most powerful tool for the rapid detection, structure elucidation, and quantification
of drug-derived material within various biological fluids