Sesamum Indicum and Linum Usitatissimum Extract on FGF and Pancreatic Histopatology White Male Mice

Abstract

Research on the effect of lignan compounds from Linumusitatissimium L.
(flaxseed) and Sesamum indicumL. (sesame) extracts had been done, combined with
Fibroblast Growth Factor (FGF) that found in egg white powder derived from fertilized egg
white. Before the research was carried out, all of the test animals’ pancreases were
damaged by alloxan given at a dose of 150 mg/kg for the positive control and test
compound preparation, except for the negative controls. Test animals were divided into 5
treatment groups named; negative control group, positive control group, and 3 test
preparation groups. The seeds’ extracts were given at a dose of 800 mg/kg and sesame
seed extract at a dose of 15 g/kg with FGF in egg white powder with dosages variations of
200, 400 and 800 mg/kg. Tests were carried out for 21 days, blood glucose levels were
checked at 7th; 14th; and 21st day. Pancreatic preparations were done by fixation,
dehydration, dipping and cutting. The results showed that in all test groups there was a
decrease in blood glucose levels compared to the positive controls. The extracts
combination with a dose of 800 mg/kg BB FGF was the optimum dose because the blood
glucose levels was decreased from 7th, 14th and 21st day (p <0.05). The results of
histopathologic examination showed that there was a fairly clear improvement of
pancreatic β cells.