Determination Method of Disperse Blue 1 in Workplace Air

Abstract

The first stage to reduce the workers' exposure to the chemicals is a detailed
identification of substances in workplaces. The aim of the study was to develop a method
for determining the concentration of carcinogenic Disperse Blue 1 in workplace air.
The method consists of drawing the air that contains Disperse Blue 1 through a cellulose
filter, washing out the substance adsorbed on the filter with methanol and analyzing the
obtained solution using high performance liquid chromatography with a diode array
detector. The determination was carried out in the reverse-phase system (mobile phase:
acetonitrile: ammonium acetate solution, 45:55) using an Ultra C18 column.
The measurement range was 0.01 - 0.2 mg/m3 for a 720 L air sample. Limit of detection
and limit of quantification were 0.52 ng/mL and 1.6 ng/mL, respectively.
The method could be useful for determining occupational exposure to this substance in the
working environment.