Preventive Effect of Mosinone-A on Immunhistochemical Expression of VEGF Marker during 7, 12-dimethylbenz (a) Anthracene Induced Hamster Buccal Pouch Carcinogenesis

Abstract

Aim: The present study was investigated to evaluate immunohistochemical
expression studies in DMBA induced hamster buccal pouch carcinogenesis.Materials and
methods: A total number of 40 golden Syrian hamsters were randomized into 4 groups of 10
animals in each. Group I animals were served as untreated control. Groups II and III animals
were painted with 0.5% DMBA in liquid paraffin three times per week for 14 weeks on the left
buccal pouches. Group III animals were orally administered with Mosinone-A (2 mg kg-1b.wt)
starting one week before the exposure to the carcinogen and continued on days alternate to
DMBA painting, until the sacrification of the animals. Group IV animals were received
Mosinone-A alone throughout the experimental period. Results: Topical application of DMBA
for 14 weeks induced buccal pouch carcinomas associated with increased expression of, VEGF,
Oral administration of Mosinone-A significantly inhibited the development of HBP carcinomas
as revealed by decreased expression of, VEGF, Conclusion: The result of the present study
indicates that Mosinone-A can exerts protective effects against DMBA induced buccal pouch
carcinogenesis. These findings suggest that Mosinone-A exerts its anticancer properties by
inhibiting cell proliferation and inducing differentiation and apoptosis.