EVALUATION OF POLYPHENOLIC PROFILE AND ANTIOXIDANT ACTIVITY OF SOME SPECIES CULTIVATED IN THE REPUBLIC OF MOLDOVA

Abstract

The aim of this study was to assess the polyphenolic composition and antioxidant
capacity of the medicinal plants from the collection of the Scientific Center of Medicinal Plants
Cultivation of Nicolae Testemitanu State University of Medicine and Pharmacy: agrimony -
Agrimonia eupatoria L. (Rosaceae), chicory - Cichorium intybus L. (Asteraceae.), artichoke -
Cynara scolymus L. (Asteraceae) and St John's-wort - Hypericum perforatum L.
(Hypericaceae). A qualitative and quantitative characterization of the main phenolic
compounds from ethanolic extracts was carried out by HPLC method. The total polyphenolic
and flavonoid contents were measured by spectrophotometric methods. The in vitro
antioxidant activity of these extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl
(DPPH), Trolox (6-hydroxy-2,5,7,8- tetramethylchroman-2-carboxylic acid) equivalent
antioxidant capacity (TEAC), 2,2′-azinobis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS)
radical cation scavenging and metal chelating activity. Considering the obtained results, the
following order in antioxidant activities was established: A. eupatoria> C. inthybus> H.
perforatum> C. scolymus. All species contain significant amounts of polyphenolic compounds
and they may be considered an important source of natural antioxidants for pharmaceutical
uses.