Preconcentration and Determination of Ciprofloxacin with Solidphase Microextraction and Silica-coated Magnetic Nanoparticles Modified with Salicylic Acid by UV-Vis Spectrophotometry

Abstract

In the present age, the pharmaceutical application continues to increase and this
results in the environmental pollution. As a result, pharmaceutical control is a common
technique in many labs. This project is on the improvement of the procedure to
determine a small amount of ciprofloxacin (CF) in bio-environmental sample.
Regarding the fact that each method requires preliminary measures to preconcentrate
and prepare analyte prior to measurement and recognition. In recent study,
preconcentration of samples containing CF (as antibiotic) based on solid-phase
microextraction method using silica-coated magnetic nanoparticles modified with
salicylic acid (SA) was proposed which acted as sorbent. In addition, UV-Vis
spectrophotometry was carried out to determine the amount of CF. The wavelength of
maximum absorption (λmax) for this drug was 275 nm. Also, the optimum conditions of
various parameters such as pH of solution, amount of magnetic nanoparticles, time,
the effect of stirring speed on the extraction and desorption, the rate of centrifugation,
the volume of aqueous and organic-phases, temperature and salt effect were
investigated. The dynamic range was obtained between 0.05-0.15 mg L-1, LOD and RSD
(%) were 0.002 mg L-1 and 1.878 respectively. Results showed using this procedure
increased the concentration factor to 68.36. Relative recovery was achieved by spike
method.