Determination of Carbendazim and Chlorpyrifos in Selected Fruits and Vegetables Samples Using QuEChERSHPLC-FD

Abstract

A new method was developed to determine pesticide residues in fruit and vegetable
extracts with short time analysis. An optimum results were achieved using binary mobile
phase consisting of (methanol: water; 95:5, v/v) and fluorescence detection (λEx and λEm set
at 280 and 340 nm, respectively) was used. The dynamic range was between 0.100 to 10
mg L-1 with relative standard deviation less than 0.45%, (n=4). Limits of detection and
recoveries for carbendazim and chlorpyrifos were 0.073 mg L-1
(84.2-106.5%) and 0.062 mg
L
-1
(85.7-90.3%), respectively. The results revealed that the concentrations of carbendazim
and chlorpyrifos residue in all collected samples were below than the EU legal limit.