Evaluation of the Mechanical Characteristics of PLGA and PLGA/fibrin Scaffolds in Providing an Appropriate Environment for Viability and Growth of Human Adipose-Derived Stem Cells

Abstract

Tissue engineered from mesenchymal stem cells (MSCs) requires a scaffold to
keep the cells in the area defect and to act as a supporter for inducing tissue formation. We
developed a three-dimensional (3-D) special poly-lactic-glycolic acid (PLGA) /fibrin (F)
composite scaffold that provided structural supporter and stimulated repair. 3-D PLGA
scaffold have been prepared via Solvent casting/salt leaching (SC/SL) techniques and the
hybrid scaffold of PLGA/F was fabricated by F. Characterization techniques such as X-ray
diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Scanning electron
microscopy (SEM) were performed. Furthermore, mechanical properties of the PLGA and
PLGA/F composite scaffolds were determined. 3-DPLGA and PLGA/F scaffolds seeded with
cultured human adipose tissue-derived stem cells (hADSCs) and analyzed by MTT at 3 and
7 days. The results revealed that the scaffolds contain sufficient porosity with highly
interconnected pore morphology. Increase the amount of fibrin enhanced compressive
modulus and compressive strength of the PLGA scaffolds. The water absorption capacity
difference for the PLGA scaffold with and without F. Our findings showed that PLGA/F
scaffold in vitro, enhanced cellular viability was observed compared to PLGA scaffold in 3
and 7 days.