Validated Spectrofluorimetric Method for The Determination of Oxymetazoline Hydrochloride via Derivatization with 4-Chloro-7- nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

Abstract

Objectives: An accurate and precise spectrofluorimetric method was developed and
validated for the determination of non-selective adrenergic drug named;
oxymetazoline hydrochloride (OMZ) in its pure form and nasal drops. Method: Based
on nucleophilic substitution reaction of target drug with 4-chloro-7-nitrobenzo-2-oxa1,3-diazole (NBD-Cl) to form a highly fluorescent fluorophore with emission measured
at 543 nm after excitation at 400 nm. Results: Under optimum conditions, the
proposed method obeys Beer’s law in the range of (1-12 µg mL-1) and the reaction
mechanism was presented. Conclusion: The method was validated according to ICH
guidelines for accuracy, precision and was successfully applied for the determination
of the drug in its pure form and nasal drops. The obtained results were statistically
compared with those of the reported method and found to be in good agreement.